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Forkhead box C1 is targeted by microRNA-133b and promotes cell proliferation and migration in osteosarcoma

Lu Deng1, Tang Liu2, Beibei Zhang1

  • 1Mental Health Institute, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China.

Experimental and Therapeutic Medicine
|September 16, 2017
PubMed

Insights

Forkhead box C1 (FOXC1) promotes osteosarcoma (OS) cell growth and migration. This oncogene is upregulated in OS and targeted by the tumor-suppressive microRNA (miR)-133b, suggesting FOXC1 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Forkhead box C1 (FOXC1) is an oncogene in various cancers.
  • The role of FOXC1 in osteosarcoma (OS) pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the expression and regulatory role of FOXC1 in osteosarcoma.
  • To explore the relationship between FOXC1 and microRNA-133b (miR-133b) in OS.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blot analysis were used to assess FOXC1 expression.
  • Cell proliferation and migration assays were performed following FOXC1 knockdown and overexpression.
  • The interaction between miR-133b and FOXC1 was investigated.

Main Results:

  • FOXC1 was significantly upregulated in OS tissues and cell lines compared to normal controls.
  • FOXC1 expression levels correlated with advanced clinical stages of OS.
  • FOXC1 knockdown inhibited OS cell proliferation and migration, while overexpression promoted these processes.
  • FOXC1 was identified as a direct target of miR-133b, with inverse correlation observed between their expressions in OS.

Conclusions:

  • FOXC1 acts as an oncogene in osteosarcoma, promoting cell proliferation and migration.
  • FOXC1 is negatively regulated by the tumor-suppressive miR-133b in OS.
  • FOXC1 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
forkhead box C1microRNAoncogeneosteosarcoma

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