Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.4K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.4K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of age on the genetic and clinical characteristics of retinitis pigmentosa.

Frontiers in ophthalmology·2026
Same author

Mucosal-associated invariant T cells recognize a tumor-derived metabolite in the DNA synthesis pathway.

Frontiers in immunology·2026
Same author

Risk factors for adverse events or use of high-potency corticosteroids in non-infectious uveitis: a Japan claims database study (J-CAT2).

Immunological medicine·2026
Same author

Protocol for the SACLA trial: Efficacy and safety of subretinal monteplase for submacular hemorrhage in a phase II single-arm multicenter decentralized clinical trial.

PloS one·2026
Same author

Myd88 Deficiency Accelerates Retinal Degeneration and Alters Microglial Dynamics in a Mouse Model of Retinitis Pigmentosa.

Investigative ophthalmology & visual science·2026
Same author

Intratarsal Keratinous Cyst Misdiagnosed as a Chalazion Complicated by an Intralesional Triamcinolone Injection: A Case Report.

Cureus·2026

Related Experiment Video

Updated: Feb 22, 2026

Intravitreal Injections in the Ovine Eye
03:37

Intravitreal Injections in the Ovine Eye

Published on: July 5, 2022

4.1K

Periostin in vitreoretinal diseases.

Shigeo Yoshida1, Takahito Nakama2, Keijiro Ishikawa2

  • 1Department of Ophthalmology, Kyushu University Graduate School of Medical Sciences, Fukuoka, 812-8582, Japan. usyosi@gmail.com.

Cellular and Molecular Life Sciences : CMLS
|September 16, 2017
PubMed
Summary

Periostin is a key molecule in proliferative vitreoretinal diseases like diabetic retinopathy. Blocking periostin effectively inhibited fibrovascular membrane formation, offering a promising new therapeutic target.

Keywords:
Age-related macular degenerationChoroidEpiretinal membranesFibrosisFibrovascular membranesGenome-wide gene expression profilingMouse model of laser-induced choroidal neovascuarizationMouse model of oxygen-induced retinal neovascularizationNeovascularizationProliferative diabetic retinopathyProliferative vitreoretinopathyRetinaSingle-stranded RNA interferenceVitreoretinal disease

More Related Videos

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
07:13

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Published on: May 13, 2014

13.9K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.5K

Related Experiment Videos

Last Updated: Feb 22, 2026

Intravitreal Injections in the Ovine Eye
03:37

Intravitreal Injections in the Ovine Eye

Published on: July 5, 2022

4.1K
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
07:13

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Published on: May 13, 2014

13.9K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.5K

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Pathology

Background:

  • Proliferative vitreoretinal diseases (PVR) cause vision loss.
  • Retinal fibrovascular membrane (FVM) formation is central to these diseases.
  • Periostin is upregulated in human FVMs and patient vitreous.

Purpose of the Study:

  • To investigate the role of periostin in FVM formation.
  • To evaluate periostin as a therapeutic target for proliferative vitreoretinal diseases.

Main Methods:

  • Gene expression profiling of human FVMs.
  • Immunohistochemistry for periostin, α-SMA, and M2 macrophage markers.
  • In vitro studies blocking periostin with PVR vitreous and TGF-β2.
  • In vivo studies using an RNAi agent targeting periostin in experimental FVM models.

Main Results:

  • Periostin expression was significantly increased in FVMs and vitreous of patients with PVR and diabetic retinopathy.
  • Periostin co-localized with α-SMA and M2 macrophage markers in FVMs.
  • Periostin blockade inhibited cell migration and adhesion in vitro.
  • Targeting periostin with RNAi reduced experimental FVM formation in vivo without retinal cell toxicity.

Conclusions:

  • Periostin is a critical mediator of FVM formation in proliferative vitreoretinal diseases.
  • Periostin represents a promising therapeutic target for conditions like diabetic retinopathy and PVR.