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Related Experiment Videos

Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization.

Meenakshi Santra1, Hong Wang2, Scott Seifert2

  • 1Department of Soil and Crop Science, Colorado State University, 307 University Ave., Fort Collins, CO, 80523, USA. meenakshi.santra@colostate.edu.

Methods in Molecular Biology (Clifton, N.J.)
|September 16, 2017
PubMed
Summary

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Doubled haploid technology enables rapid development of homozygous wheat lines in 1-2 years, significantly reducing cultivar development time compared to traditional breeding methods. This advanced technique utilizes wheat-maize wide hybridization for efficient production of uniform breeding lines.

Area of Science:

  • Plant breeding
  • Genetics
  • Agricultural biotechnology

Background:

  • Traditional wheat breeding relies on multiple generations of selfing to achieve homozygosity.
  • This process is time-consuming, delaying the development of uniform crop lines.

Purpose of the Study:

  • To highlight the efficiency of doubled haploid technology in accelerating wheat cultivar development.
  • To detail the primary methods and critical factors for successful doubled haploid production in wheat.

Main Methods:

  • Utilizes wheat-maize wide hybridization, involving emasculation, pollination with maize, hormone treatment, embryo rescue, and chromosome doubling.
  • Androgenesis (anther or microspore culture) is an alternative method for producing haploids.

Main Results:

Keywords:
Doubled haploidEmbryo rescueHomozygousWheatWide hybridization

Related Experiment Videos

  • Doubled haploid technology achieves 100% homozygosity in a single generation, yielding completely homogeneous breeding lines.
  • This method significantly reduces the time required for cultivar development to 1-2 years.

Conclusions:

  • Wheat-maize wide hybridization is the most effective and widely used method for rapid production of homozygous lines.
  • Efficiency is influenced by wheat and maize genotypes, plant health, greenhouse conditions, and procedural precision, necessitating potential modifications for optimization.