Transient transfection of Cryptosporidium baileyi

Here we demonstrate the transient transfection of C. baileyi. We describe the transfection of this apicomplexan parasite and the cultivation in ovo. The functionality of heterologous sequences in C. baileyi was demonstrated by the expression and detection of the mCherry protein in ovo. The mCherry protein was expressed in parasitic stages up to the oocyst stage under the control of the heterologous promoter region of the C. parvum actin gene.