Related Experiment Video
Updated: Feb 22, 2026

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Tracking and controlling soft surface contamination in health care settings
Jonathan D Sexton1, Amanda M Wilson1, Hannah P Sassi1
1Mel & Enid Zuckerman College of Public Health, The University of Arizona, Tucson, AZ.
Background:
Study objectives were to track the transfer of microbes on soft surfaces in health care environments and determine the efficiency of an Environmental Protection Agency (EPA)-registered soft surface sanitizer in the health care environment.
Methods:
Soft surfaces at 3 health care facilities were sampled for heterotrophic plate count (HPC) bacteria, Staphylococcus spp, Streptococcus pyogenes, and Escherichia coli followed by a tracer study with a virus surrogate seeded onto volunteer hands and commonly touched surfaces. The occurrence of microbial contaminants was determined along with microbial reductions using the soft surface sanitizer. Soft surfaces were swabbed pre- and postintervention.
Results:
Tracer viruses spread to 20%-64% and 13%-41% of surfaces in long-term health care facilities and physicians' offices, respectively. Only 1 pathogen, methicillin-resistant Staphylococcus aureus, was recovered. The waiting room chairs had the highest concentration of HPC bacteria before disinfection (145.4 ± 443.3 colony forming units [cfu]/cm2), and the privacy curtains had the lowest (39.5 ± 84.2 cfu/cm2). Reductions of up to 98.5% were achieved with the sanitizer in health care settings and up to 99.99% under controlled laboratory conditions.
Conclusions:
Soft surfaces are involved in the spread of microbes throughout health care facilities. Routine application of an EPA-registered sanitizer for soft surfaces can help to reduce the microbial load and minimize exposure risks.
More Related Videos
05:49Tracking Microbial Contamination in Retail Environments Using Fluorescent Powder - A Retail Delicatessen Environment Example
Published on: March 5, 2014
07:00Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface
Published on: August 11, 2017
Related Concept Videos
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Healthcare Associated Infections II: Preventive Measures
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Hand hygiene
Hand washing...
Methods for Controlling Microbial Growth
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Asepsis