A comparison study between electrospun polycaprolactone and piezoelectric...

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Materials Science

Background:

• Developing effective bone scaffolds is crucial for bone tissue engineering.
• Polycaprolactone (PCL) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are common biomaterials.
• Incorporating piezoelectric properties and nanoparticles can enhance scaffold performance.

Purpose of the Study:

• To fabricate and characterize novel bone scaffolds using PCL, PHBV, and PHBV-SiHA.
• To evaluate the influence of piezoelectricity and SiHA nanoparticles on scaffold properties.
• To assess the in vitro biological performance of these scaffolds with human mesenchymal stem cells (hMSCs).

Main Methods:

• Electrospinning was used to fabricate PCL, PHBV, and PHBV-SiHA scaffolds.
• Scaffold morphology, fiber diameter, and surface roughness were analyzed.
• Chemical composition and wetting behavior were determined using XRD, FTIR, Raman spectroscopy, and contact angle measurements.
• In vitro studies assessed hMSC adhesion, viability, spreading, and osteogenic differentiation.

Main Results:

• Scaffolds exhibited randomly oriented fibers with diameters ranging from 800nm to 12μm.
• Addition of SiHA increased fiber size and surface roughness.
• PHBV-SiHA scaffolds demonstrated superior hMSC adhesion, spreading, and osteogenic differentiation.
• Piezoelectric PHBV scaffolds showed enhanced calcium deposition compared to non-piezoelectric PCL.

Conclusions:

• Hybrid PHBV-SiHA scaffolds possess significant advantages for bone tissue engineering.
• The combination of piezoelectricity and SiHA nanoparticles enhances cellular response.
• These novel scaffolds show promise for promoting bone regeneration.