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En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
Dihydropyridine-derived BODIPY probe for detecting exogenous and endogenous nitric oxide in mitochondria
Chang Gao1, Lan Lin2, Wan Sun3
1National Institute for Food and Drug Control, Institute of Chemical Drug Control, TianTan XiLi 2, Beijing 100050, China; College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China; School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
Abstract:
A mitochondria-targetable probe Mito-DHP for nitric oxide (NO) was designed and synthesized by introducing dihydropyridine and triphenylphosphonium (TPP) moieties into boron dipyrromethene (BODIPY) dye. Mito-DHP was able to effectively detect nitric oxide through the aromatization of dihydropyridine to fluorescent pyridine product under oxygen-free conditions. The probe Mito-DHP showed high selectivity to NO over a number of reactive oxygen/nitrogen species (ROS/RNS) as well as high sensitivity (detection limit at 25nM), pH stability and bio-compatibility. Furthermore, Mito-DHP proved to target mitochondria specifically and to visualize both exogenous and endogenous NO in real time.
