Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K
Labeling DNA Probes03:31

Labeling DNA Probes

9.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aromatic amine nitration mechanisms: reactivity governed by resonance-assisted hydrogen bonds.

Physical chemistry chemical physics : PCCP·2026
Same author

Exploring the Correlation between Azido-Tetrazolo Tautomerizations and Isomer Structures: Electron Density of Bonding N Atoms and N-N Bond Polarity.

The journal of physical chemistry. A·2024
Same author

Exploring the thermal decomposition and detonation mechanisms of 2,4-dinitroanisole by TG-FTIR-MS and molecular simulations.

RSC advances·2024
Same author

How are <i>N</i>-methylcarbamates encapsulated by β-cyclodextrin: insight into the binding mechanism.

Physical chemistry chemical physics : PCCP·2023
Same author

A single-wavelength excited NIR fluorescence probe for distinguishing GSH/H<sub>2</sub>S and Cys/Hcy in living cells and zebrafish through separated dual-channels.

Talanta·2022
Same author

Evaluating the impact of Hartree-Fock exact exchange on the performance of global hybrid functionals for the vertical excited-state energies of fused-ring electron acceptors using TD-DFT.

Physical chemistry chemical physics : PCCP·2022

Related Experiment Video

Updated: Feb 22, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
06:33

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Published on: July 5, 2021

2.4K

Fluorescence quenching based alkaline phosphatase activity detection.

Yaqi Mei1, Qiong Hu1, Baojing Zhou2

  • 1School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China.

Talanta
|September 18, 2017
PubMed
Summary

A new fluorescence quenching method enables rapid, real-time detection of alkaline phosphatase (ALP) activity. This approach uses a charge transfer mechanism with the Cu(BCDS)22- probe for sensitive diagnostics and drug discovery.

Keywords:
Alkaline phosphatase (ALP)Bathocuproine disulfonateCharge transferDensity functional theoryFluorescence quenchingFluorometric assay

More Related Videos

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

10.4K
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

16.4K

Related Experiment Videos

Last Updated: Feb 22, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
06:33

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Published on: July 5, 2021

2.4K
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

10.4K
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

16.4K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Simple and fast detection of alkaline phosphatase (ALP) activity is crucial for diagnostics.
  • Existing methods may lack speed, sensitivity, or require complex probes.

Purpose of the Study:

  • To develop a novel turn-off fluorescence approach for real-time ALP activity detection.
  • To utilize a charge transfer mechanism for sensitive and selective ALP sensing.

Main Methods:

  • Employed a Cu(BCDS)22- probe that undergoes fluorescence quenching via charge transfer.
  • Utilized ascorbic acid 2-phosphate as a substrate, releasing ascorbic acid (AA) upon ALP hydrolysis.
  • Validated the mechanism using quantum mechanical calculations.

Main Results:

  • Achieved real-time, sensitive detection of ALP activity (0-220mUmL-1, LOD 0.27mUmL-1).
  • Demonstrated high selectivity and robustness against photo bleaching.
  • Successfully applied the method to undiluted human serum and whole blood samples.

Conclusions:

  • The charge transfer-based fluorescence quenching method offers a fast, simple, and sensitive platform for ALP detection.
  • This approach has significant potential for diagnostic applications, drug discovery (inhibitor screening), and biological sample analysis.