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Related Experiment Video

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Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
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Rapid Adenovirus typing method for species identification.

Fabienne Rayne1, Linda Wittkop2, Clément Bader3

  • 1Univ Bordeaux, CNRS, UMR 5234 Fundamental Microbiology and Pathogenicity, F-33000, Bordeaux, France.

Journal of Virological Methods
|September 18, 2017
PubMed
Summary

A new rapid typing method accurately identifies Adenovirus species A and C, which are linked to severe illness. This cost-effective technique aids in clinical diagnosis for both immunocompetent and immunocompromised patients.

Keywords:
AdenovirusRapid methodSpecies typingpVI

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Area of Science:

  • Virology
  • Clinical Microbiology

Background:

  • Adenoviruses exhibit significant diversity, classified into species A-G.
  • Certain species, like A and C, are associated with increased clinical severity in various patient groups.

Purpose of the Study:

  • To develop a rapid, simple, and cost-effective method for Adenovirus species identification.
  • To assess the clinical relevance of Adenovirus species typing.

Main Methods:

  • A novel "pVI rapid typing method" was designed.
  • This method utilizes combined fusion temperature (Tm) and amplicon size analysis.
  • Results were compared to traditional Sanger sequencing of the hexon gene in 140 clinical samples.

Main Results:

  • The pVI rapid typing method demonstrated high accuracy for Adenovirus species identification.
  • Species A and C were identified with 100% positive predictive value.
  • The method proved to be quick, simple, and cost-effective.

Conclusions:

  • The pVI rapid typing method is a valuable tool for rapid Adenovirus species assignment.
  • Species identification, particularly for A and C, provides clinically relevant information.
  • This method can aid in timely and accurate clinical diagnosis and management.