High sensitivity method validated to quantify estradiol in human plasma by LC-MS/MS

Area of Science:

• Endocrinology
• Analytical Chemistry
• Biomarker Quantification

Background:

• 17β-Estradiol (E2) is a vital endogenous steroid hormone.
• Accurate E2 measurement in human plasma is essential for understanding health and human biology.
• Low physiological E2 concentrations (pg/mL) present significant analytical challenges.

Purpose of the Study:

• To validate a highly sensitive and accurate LC-MS/MS method for quantifying 17β-estradiol in human plasma.
• To establish a reproducible assay suitable for measuring low E2 levels.

Main Methods:

• Development and validation of a novel sample extraction technique to improve chromatographic purity.
• Utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS) for precise E2 quantification.
• Assessed E2 stability under various storage and handling conditions.

Main Results:

• The developed method achieved high sensitivity and accuracy for E2 quantification in human plasma.
• The assay demonstrated excellent precision and reproducibility within the validated range of 2-150 pg/mL.
• Long-term stability of E2 was confirmed under tested conditions, indicating method robustness.

Conclusions:

• The validated LC-MS/MS method is robust, sensitive, and accurate for measuring low 17β-estradiol concentrations in human plasma.
• This method is suitable for application in bioequivalence studies requiring precise E2 quantification.