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Updated: Feb 22, 2026

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

Improving membrane protein expression by optimizing integration efficiency.

Michiel J M Niesen¹, Stephen S Marshall¹, Thomas F Miller²

¹From the Department of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125.

The Journal of Biological Chemistry

|September 18, 2017

Summary

This summary is machine-generated.

Improving integral membrane protein expression in E. coli is crucial. This study uses simulated membrane integration efficiency to predict sequence modifications that enhance protein overexpression, enabling rational protein engineering.

Keywords:

Sec translocon TatC flow cytometry membrane protein molecular dynamics protein expression protein translocation topogenesis

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Area of Science:

Biochemistry
Computational Biology
Molecular Biology

Background:

Heterologous overexpression of integral membrane proteins in Escherichia coli often results in low yields.
Integral membrane protein expression is critical for various biotechnological and research applications.

Purpose of the Study:

To develop a predictive method for enhancing integral membrane protein expression.
To investigate the link between co-translational membrane integration efficiency and protein expression levels.

Main Methods:

Utilized coarse-grained simulations to calculate membrane integration efficiencies for protein sequence modifications.
Introduced 140 sequence modifications (mutations and chimeras) to the integral membrane protein TatC.
Experimentally validated the effects of mutations on protein expression levels.

Main Results:

Simulated membrane integration efficiencies accurately predicted experimental expression levels for TatC.
Mutations improving simulated integration efficiency were 4-fold enriched for improved experimental expression.
The effects of multiple mutations on expression were cumulative and largely independent.

Conclusions:

Computationally simulated membrane integration efficiency can guide rational engineering of integral membrane proteins.
This approach offers a general strategy for improving the overexpression of challenging membrane proteins.
The findings facilitate the production of higher yields of purifiable membrane proteins for diverse applications.