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Updated: Feb 22, 2026

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
Published on: June 20, 2019
Determination of molar masses of macromolecules by size exclusion chromatography-light scattering not requiring
Simona Lavric1, Jasmin Preis2, Christine Rosenauer3
1Melamin d.d., Kocevje, Slovenia.
Abstract:
A new approach for the calibration of SEC-light scattering (SEC-LS) setups is proposed, which requires solely the molar mass of a reference polymer. Neither the specific refractive index increment of the calibrant nor of the analyte is required. Comparison of the molar masses derived in different solvents for a large number of chemically different polymers shows that the new approach yields the same molar masses as if molar masses were derived using dn/dc to calibrate the light scattering setup. The approach therefore allows easier determination of molar masses by SEC-LS.
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