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Updated: Feb 22, 2026
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes
Hongguo Cao1,2, Yani Bian1, Fei Zhang1
1College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Objective:
cAMP and mature promoting factor (MPF) play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes (DOs) in mice by regulating cAMP and MPF.
Methods:
In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during in vitro maturation without follicle stimulating hormone and luteinizing hormone, respectively.
Results:
Combined use of 50 μM Forskolin for 3 h and 2.5 μM PD166285 for additional 21 h enhanced the developmental competence of DOs, maturation rate of DOs was 76.71%± 4.11%, blastocyst rate was 18.33%±4.44% after parthenogenetic activation (PA). The DOs could successfully be fertilized with sperm in vitro, cleavage rate was 17.02%±5.82% and blastocyst rate was 5.65%±3.10%. Besides, 2-cell in vitro fertilization embryos from DOs produced 4 normal live offspring (4/34).
Conclusion:
The results confirmed that the combination of Forskolin and PD166285 can induce DOs to complete meiosis process and produce normal offspring.
