Ramlibacter alkalitolerans sp. nov., alkali-tolerant bacterium isolated from soil of ginseng

A novel bacterial strain, designated CJ661^T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661^T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661^T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661^T showed that it belongs to the genus Ramlibacter within the family Comamonadaceae and was most closely related to Ramlibacter ginsenosidimutans KCTC 22276^T (98.1 %), followed by Ramlibacter henchirensis DSM 14656^T (97.1 %). DNA-DNA relatedness levels of strain CJ661^T were 40.6 % to R. ginsenosidimutans KCTC 22276^T and 25.0 % to R. henchirensis DSM 14656^T. The major isoprenoid quinone was ubiquinone (Q-8). The predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids of strain CJ661^T were summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The G+C content of the genomic DNA was 65.4 mol%. On the basis polyphasic taxonomic data, strain CJ661^T represents a novel species in the genus Ramlibacter, for which name Ramlibacter alkalitolerans sp. nov. is proposed; the type strain is CJ661^T (=KACC 19305^T=JCM 32081^T).