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A method for determining polysaccharide content in biological samples.
Duoduo Xu1, Wei Zheng2, Yanqiu Zhang3
1Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin 130117, China; Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun 130117 Jilin, China.
International Journal of Biological Macromolecules
|September 20, 2017
Summary
This study introduces a novel method to measure polysaccharide content in biological samples using an iodine complex and ICP-MS. This technique enhances the study of polysaccharide pharmacokinetics and bioavailability in vivo.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Pharmacokinetics
Background:
- Determining polysaccharide content in biological samples is challenging due to complex structures, hindering pharmacokinetic studies.
- Existing methods face limitations in simplicity, reproducibility, and reliability for biological matrices.
Purpose of the Study:
- To develop a simple, reproducible, and reliable method for quantifying polysaccharide content in biological samples.
- To assess the stability and in vivo application of a novel polysaccharide quantification method.
Main Methods:
- Polysaccharides were complexed with fluorescein isothiocyanate (FITC) and iodine.
- Iodine content in the complex was measured using inductively coupled plasma-mass spectrometry (ICP-MS) for indirect polysaccharide quantification.
- Methodological validation and stability studies were performed in vitro and in vivo.
Main Results:
- The developed method demonstrated stability of the iodine-polysaccharide complex both in vitro and in vivo.
- Oral administration of iodine-substituted polysaccharides in rats showed the highest blood absorption rate at 1 hour (5.84%).
- Bioaccessibility varied across organs, with an average of 1% in organs after 3 hours.
Conclusions:
- The ICP-MS based method using an iodine complex provides a reliable approach for polysaccharide content determination in biological samples.
- This method facilitates the investigation of polysaccharide pharmacokinetics and bioavailability.
- The findings support the utility of this technique for future research in polysaccharide-based drug development.
