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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

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Immunoadsorption using Immusorba TR and PH.

Ryuichiro Hirano¹, Noriko Hirata²

¹International Disposable Product Marketing Department, Asahi Kasei Medical Co., Ltd., Japan.

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis

|September 20, 2017

Summary

Immusorba TR and PH are nonbiological immunoadsorbents using tryptophan or phenylalanine ligands to remove pathogenic substances. These treatments target autoimmune neurological and rheumatic diseases, aiding in disease pathogenesis research.

Keywords:

Autoantibody Immunoadsorbent Phenylalanine Polyvinyl alcohol gel Tryptophan

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Area of Science:

Biomaterials Science
Immunology
Clinical Therapeutics

Background:

Autoimmune diseases involve pathogenic substances that can be targeted for removal.
Nonbiological immunoadsorbents offer an alternative to biological treatments.
Immusorba TR (IM-TR) and Immusorba PH (IM-PH) were developed as novel immunoadsorbent therapies.

Purpose of the Study:

To describe the development and application of Immusorba TR and Immusorba PH.
To highlight their efficacy in treating autoimmune neurological and rheumatic diseases.
To explore their potential in identifying unknown pathogenic substances and elucidating disease mechanisms.

Main Methods:

Immusorba TR and IM-PH utilize tryptophan and phenylalanine, respectively, as affinity ligands.
These ligands are immobilized on a polyvinyl alcohol gel matrix.
Clinical applications involve extracorporeal removal of pathogenic substances from patient blood.

Main Results:

IM-TR is primarily used for autoimmune neurological conditions like myasthenia gravis, Guillain-Barré syndrome, and multiple sclerosis.
IM-PH is applied to neurological and rheumatic diseases, including rheumatoid arthritis and systemic lupus erythematosus.
Autoantibodies with diverse specificities show significant affinity for Immusorba ligands.

Conclusions:

Immusorba TR and PH are effective nonbiological immunoadsorbents for treating various autoimmune diseases.
The broad affinity of Immusorba ligands suggests potential for wider therapeutic applications.
Adsorbed substances can provide insights into disease pathogenesis, facilitating the identification of unknown pathogenic factors.