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Updated: Feb 22, 2026
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
Development of EST-SSR markers for Taxillus nigrans (Loranthaceae) in southwestern China using next-generation
Ning Miao1, Lei Zhang1, Maoping Li1
1MOE Key Laboratory of Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, People's Republic of China.
Premise Of The Study:
We developed transcriptome microsatellite markers (simple sequence repeats) for Taxillus nigrans (Loranthaceae) to survey the genetic diversity and population structure of this species.
Methods And Results:
We used Illumina HiSeq data to reconstruct the transcriptome of T. nigrans by de novo assembly and used the transcriptome to develop a set of simple sequence repeat markers. Overall, 40 primer pairs were designed and tested; 19 of them amplified successfully and demonstrated polymorphisms. Two loci that detected null alleles were eliminated, and the remaining 17, which were subjected to further analyses, yielded two to 21 alleles per locus.
Conclusions:
The markers will serve as a basis for studies to assess the extent and pattern of distribution of genetic variation in T. nigrans, and they may also be useful in conservation genetic, ecological, and evolutionary studies of the genus Taxillus, a group of plant species of importance in Chinese traditional medicine.
