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Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae)
Mariana Bulgarella1, Patrick J Biggs2, Peter J de Lange3
1Museum of New Zealand Te Papa Tongarewa, P.O. Box 467, Wellington, New Zealand.
Premise Of The Study:
Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other Arthropodium species.
Methods And Results:
Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum. The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross-amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum.
Conclusions:
These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.
