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Related Experiment Video

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Endothelial precursor cell cross-match using Tie-2-enriched spleen cells.

Volker Daniel1, Caner Süsal1, Sabine Scherer1

  • 1Transplantation-Immunology, Institute of Immunology, University-Hospital Heidelberg, Heidelberg, Germany.

Clinical Transplantation
|September 20, 2017
PubMed
Summary

This study demonstrates that spleen cell-derived endothelial progenitor cells (EPCs) can be used for cross-matching, identifying pre-transplant IgG EPC antibodies in kidney recipients, which may impact graft outcomes.

Keywords:
XM-ONEantibodies against endothelial progenitor cellsdeceased-donor kidney graftspre-formed EPC antibodiesspleen cells

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Area of Science:

  • Transplantation immunology
  • Cellular immunology
  • Vascular biology

Background:

  • Non-HLA antibodies against endothelial progenitor cells (EPCs) in pre-transplant serum can negatively affect graft outcomes.
  • Peripheral blood-derived EPCs are commonly used for EPC cross-matching.
  • Expanding sample sources for cross-matching is crucial for deceased-donor and retrospective studies.

Purpose of the Study:

  • To describe and evaluate cross-matches using EPCs derived from spleen cell preparations (fresh or frozen-thawed).
  • To assess the feasibility of using spleen cell-derived EPCs for pre-transplant cross-matching.
  • To investigate the prevalence of IgG EPC antibodies in kidney transplant recipients with varying graft outcomes.

Main Methods:

  • Retrospective cross-matches were performed using spleen cells.
  • The XM-ONE cross-match test kit, a flow cytometric assay, was utilized.
  • EPCs were enriched from fresh or frozen-thawed spleen cell preparations.

Main Results:

  • Healthy controls (n=28) showed no IgG EPC antibodies.
  • Among 11 random dialysis patients, 2 (18%) had IgG EPC antibodies.
  • In kidney recipients with good long-term graft survival (n=20), 3 (15%) had pre-transplant IgG EPC antibodies using donor spleen cells.
  • Conversely, 4 out of 5 (80%) patients with intra-operative graft loss exhibited IgG EPC antibodies.

Conclusions:

  • Cross-matching using spleen cell-derived EPCs with the XM-ONE assay is technically feasible.
  • Preliminary findings suggest potential clinical relevance in predicting kidney transplant outcomes.
  • Spleen cell-derived EPCs offer a viable alternative sample source for cross-matching.