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A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles
Published on: April 10, 2019
Mediatorless Impedance Studies with Titanium Dioxide Conjugated Gold Nanoparticles for Hydrogen Peroxide Detection
Nur Hamidah Abdul Halim1,2, Yook Heng Lee3, Radha Swathe Priya Malon Marugan4
1School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600 UKM, Selangor, Malaysia. nurhamidah@unimap.edu.my.
Abstract:
An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO₂) particles for hydrogen peroxide (H₂O₂) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO₂, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-like activity, was used as the bioreceptor in the biosensor to detect H₂O₂ in solution. The analysis was carried out using an alternative impedance method, in which the biosensor exhibited a wide linear range response between 1 × 10-4 M and 1.5 × 10-2 M and a limit of detection (LOD) of 1 × 10-5 M without a redox mediator.
