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MiR-381 inhibits migration and invasion in human gastric carcinoma through downregulatedting SOX4

Mingming Zhang1, Shishu Huang2, Dan Long3

  • 1Department of Gastroenterological Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

Oncology Letters
|September 21, 2017
PubMed

Insights

microRNA-381 (miR-381) is downregulated in gastric cancer (GC), promoting metastasis by inhibiting SOX4. Restoring miR-381 levels may offer a new therapeutic strategy for GC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant microRNA (miR) expression is crucial in human cancer development and progression.
  • Gastric carcinoma (GC) is a significant global health concern, necessitating research into its underlying molecular mechanisms.

Purpose of the Study:

  • To investigate the expression and functional role of miR-381 in human gastric carcinoma.
  • To elucidate the molecular pathways regulated by miR-381 in GC progression and metastasis.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to analyze miR-381 expression levels.
  • Cellular assays were performed to assess the effects of miR-381 on GC cell migration and invasion.
  • The regulatory relationship between miR-381 and long non-coding RNA-taurine upregulated 1 (TUG1) was investigated.

Main Results:

  • miR-381 expression was significantly downregulated in GC tissues compared to normal tissues.
  • Low miR-381 expression correlated with increased lymphatic metastasis and advanced tumor-node-metastasis (TNM) stage (III+IV).
  • Upregulation of miR-381 suppressed GC cell migration and invasion by inhibiting SRY-Box 4 (SOX4)-mediated epithelial-mesenchymal transition (EMT).
  • Long non-coding RNA-TUG1 was identified as a negative regulator of miR-381 expression in GC cells.

Conclusions:

  • The downregulation of miR-381, mediated by lncRNA-TUG1, promotes GC cell metastasis through SOX4 inhibition.
  • Targeting miR-381 presents a potential novel therapeutic strategy for gastric carcinoma treatment.
Keywords:
SOX4gastric carcinomainvasionmiR-381migration

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