Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

972
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
972
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

764
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
764
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

11.7K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
11.7K
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

623
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
623
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

1.7K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.7K
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

2.0K
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive analysis of the lipid composition in Saccharomyces cerevisiae, Saccharomyces eubayanus, and Saccharomyces pastorianus grown at different temperatures by untargeted lipidomics.

Bioscience, biotechnology, and biochemistry·2026
Same author

Metabolic bottleneck at TCA cycle entry point and competition with trehalose production hinder growth of methylotrophic yeasts during methanol assimilation.

Journal of bioscience and bioengineering·2026
Same author

Fine modulation of carbon flow in central carbon metabolism via ribosome-binding site modification in <i>Escherichia coli</i>.

Metabolic engineering communications·2026
Same author

Exploration of Yeast Species Suitable for Preparation of Stable Isotope-Labeled Internal Standards Extracts (SILIS).

Mass spectrometry (Tokyo, Japan)·2025
Same author

MassBank: an open and FAIR mass spectral data resource.

Nucleic acids research·2025
Same author

<sup>13</sup>C-metabolic flux analysis of K562 cells before and after differentiation into erythroid reveals a metabolic shift toward oxidative metabolism.

Journal of bioscience and bioengineering·2025

Related Experiment Video

Updated: Feb 22, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.1K

Learning from quantitative data to understand central carbon metabolism.

Fumio Matsuda1, Yoshihiro Toya2, Hiroshi Shimizu2

  • 1Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.

Biotechnology Advances
|September 21, 2017
PubMed
Summary
This summary is machine-generated.

Quantitative metabolic analysis aids cancer research and metabolic engineering. Metabolic flux analysis is crucial for understanding cell metabolism, though regulatory mechanisms remain complex.

Keywords:
Central carbon metabolismEnzyme reaction kineticsGibbs free-energy changeMetabolic fluxSpecific rates

More Related Videos

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

10.0K
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
07:41

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Published on: February 4, 2017

9.0K

Related Experiment Videos

Last Updated: Feb 22, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.1K
Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

10.0K
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
07:41

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Published on: February 4, 2017

9.0K

Area of Science:

  • Biochemistry
  • Systems Biology
  • Metabolic Engineering

Background:

  • Quantitative analysis of cellular metabolism is vital for understanding disease mechanisms and optimizing biological processes.
  • Identifying key metabolic pathways and bottlenecks is essential in fields ranging from oncology to industrial biotechnology.

Purpose of the Study:

  • To review and compare various methods for quantitative metabolic analysis.
  • To highlight the importance of metabolic flux analysis (MFA) for quantitative investigations.
  • To discuss the role of metabolite concentrations in assessing metabolic thermodynamics.

Main Methods:

  • Comparative review of different metabolic analysis techniques.
  • Emphasis on material balance-based metabolic flux analysis.
  • Discussion of metabolite concentration determination for thermodynamic evaluation.

Main Results:

  • Metabolic flux analysis is presented as a critical method for quantitative studies of cell metabolism.
  • The determination of absolute metabolite concentrations is essential for evaluating metabolic thermodynamic states.
  • Challenges remain in elucidating regulatory mechanisms from static data due to incomplete knowledge.

Conclusions:

  • Metabolic flux analysis is a key quantitative tool for biological research.
  • Accurate metabolite quantification is necessary for understanding metabolic thermodynamics.
  • Further research is needed to fully decipher metabolic regulatory networks.