Influence of adrenergic agonists and antagonists on lymphokine secretion in vitro

Spleen cells of mice and lymph node lymphocytes of rats were cultured with epinephrine, norepinephrine and alpha- and beta-adrenergic drugs to determine effects on lymphokine secretion. After an incubation of 4.5 h it was found that the naturally occurring catecholamines and adrenergic drugs (isoprenaline, orciprenaline, terbutaline, norfenephrine, phenylephrine, clonidine, dobutamine) could induce the secretion of charge-changing lymphokines and lymphokines that stimulate the migration of lymphocytes. The beta-blocker propranolol inhibited the lymphokine secretion evoked by epinephrine and by beta-adrenergic agonists, whereas the alpha-blocker phentolamine antagonized the effects elicited by alpha-adrenergic drugs. Phenylethylamine also induced a significant secretion of lymphokines. Two non-phenylethylamines had no effect. The stimulatory capacity of adrenergic agonists, the inhibitory activity of the appropriate antagonists and the comparison with two non-phenylethylamines support the hypothesis on specific influence of catecholamines on the lymphocyte function.