Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenotypic and molecular characterization of K64 putative hypervirulent carbapenem-resistant Klebsiella pneumoniae.

PloS one·2026
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026
Same author

Probe-Based Chemical Proteomics Identifies UDP-Glucose 6-Dehydrogenase as a Potential Cannabidiol-Interacting Protein.

Journal of natural products·2026
Same author

Bronchoscope: an important and easily overlooked vector of clinical infection with <i>Klebsiella pneumoniae</i>.

Frontiers in cellular and infection microbiology·2026
Same author

Divergent toxicity mechanisms of amyloid-beta aggregates arising from a single aggregation reaction.

Cell reports·2026
Same author

Tracking Gene Expression of Single Mitochondria in Live Neurons Using Nanotweezers.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Feb 22, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

8.0K

Nanopore extended field-effect transistor for selective single-molecule biosensing.

Ren Ren1,2, Yanjun Zhang3,4, Binoy Paulose Nadappuram2

  • 1Department of Medicine, Imperial College London, London, W12 0NN, UK.

Nature Communications
|September 21, 2017
PubMed
Summary

Researchers developed a new nanopore extended field-effect transistor (nexFET) biosensor for highly selective, sensitive, and rapid detection of molecules in biological fluids, addressing key challenges in nanotechnological biosensing.

More Related Videos

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

11.7K
Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing
10:45

Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing

Published on: August 29, 2025

755

Related Experiment Videos

Last Updated: Feb 22, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

8.0K
Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

11.7K
Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing
10:45

Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing

Published on: August 29, 2025

755

Area of Science:

  • Nanotechnology
  • Biosensing
  • Transistor technology

Background:

  • Existing biosensors lack affordability, integration, speed, multiplexing, and high selectivity for trace analytes.
  • Nanotechnology offers potential solutions but faces challenges in practical biosensor development.

Purpose of the Study:

  • To address limitations in current biosensor technology by developing a novel nanoscale sensor.
  • To create a biosensor combining nanopore sensing, field-effect transistors, and recognition chemistry for enhanced performance.

Main Methods:

  • Design and fabrication of a nanopore extended field-effect transistor (nexFET) biosensor.
  • Functionalization of the nexFET with polypyrrole and an embedded receptor.
  • Utilizing controllable gate voltage to modulate single-molecule transport through the nanopore.

Main Results:

  • Demonstrated controllable DNA transport through the nanopore using gate voltage.
  • Achieved enhanced signal-to-noise ratio and molecular throughput.
  • Showcased selective detection of anti-insulin antibody in the presence of IgG isotype, indicating high selectivity.

Conclusions:

  • The developed nexFET biosensor offers a promising platform for efficient, selective, and sensitive single-molecule detection.
  • This technology addresses critical unmet needs in biosensing for biological fluid analysis.
  • The controllable gate voltage mechanism enhances sensor performance and selectivity.