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Bioactivity-based antioxidative components screening and evaluation in grape seed proanthocyanidin extract.

Jie Gao1, Shengtao Lv1, Changzhi Li1

  • 1State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, 38 Tongyan Road, Haihe Education Park, Tianjin, 300353 People's Republic of China.

Journal of Food Science and Technology
|September 21, 2017
PubMed
Summary

Grape seed proanthocyanidin extract (GSPE) protects cells from oxidative stress. Advanced analysis identified Procyanidin B2 and C2 as key antioxidant compounds within GSPE.

Keywords:
AntioxidantsFluorescent probeG-quadruplexGrape seed proanthocyanidinsOxidative stress

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Food Science

Background:

  • Grape seed proanthocyanidin extract (GSPE) is recognized for its potent antioxidant properties.
  • Oxidative stress, often induced by hydrogen peroxide (H₂O₂), can cause significant cellular damage.
  • Understanding the specific active components in GSPE is crucial for harnessing its health benefits.

Purpose of the Study:

  • To identify the specific compounds in GSPE responsible for its antioxidant activity.
  • To investigate the protective effects of GSPE against H₂O₂-induced oxidative stress in human embryonic kidney 293 (HEK 293) cells.
  • To validate the efficacy of novel analytical techniques for screening bioactive compounds.

Main Methods:

  • Utilized a modified ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS) system integrated with an ABTS radical cation antioxidative activity analysis system.
  • Employed a novel N-borylbenzyloxycarbonyl-3,7-dihydroxyphenoxazine (NBCD) fluorescent probe for in situ molecular imaging of H₂O₂ levels.
  • Assessed the dose-dependent protective effects of GSPE on HEK 293 cells against H₂O₂-induced injury.

Main Results:

  • GSPE demonstrated a dose-dependent protection of HEK 293 cells against H₂O₂-induced cell injury and oxidative stress.
  • Procyanidin B2 and Procyanidin C2 were identified as the primary constituents exhibiting significant antioxidant activity within GSPE.
  • The study successfully visualized the H₂O₂ scavenging effect of Procyanidin B2 in real-time using the NBCD fluorescent probe.

Conclusions:

  • The integrated UPLC-Q/TOF MS and ABTS system is effective for screening antioxidant components in functional foods like GSPE.
  • Procyanidin B2 and Procyanidin C2 are key active compounds contributing to the antioxidant capacity of GSPE.
  • The NBCD fluorescent probe provides a valuable tool for visualizing and confirming the in situ antioxidant activity of compounds like Procyanidin B2.