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Updated: Feb 22, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

Dong-Dong Cheng1, Bin Zhu1, Shi-Jie Li1

  • 1Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

Journal of Cancer
|September 21, 2017
PubMed

Insights

Ribosomal protein S9 (RPS9) is elevated in osteosarcoma, promoting tumor growth and progression. Inhibiting RPS9 may offer a new therapeutic strategy for this bone cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma is a prevalent adolescent bone cancer with incompletely understood pathogenesis.
  • Ribosomal protein S9 (RPS9) has been implicated in the tumorigenesis of various cancers.

Purpose of the Study:

  • To investigate the expression, function, and underlying mechanisms of RPS9 in human osteosarcoma.
  • To determine RPS9's role in osteosarcoma cell proliferation, cell cycle, and associated signaling pathways.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and western blotting assessed RPS9 expression in osteosarcoma tissues and cell lines.
  • RNA interference (RNAi) was used to knock down RPS9 in MNNG/HOS, MG63, and U2OS cell lines.
  • Intracellular signaling antibody array identified pathways affected by RPS9 knockdown.

Main Results:

  • RPS9 expression was significantly upregulated in osteosarcoma tissues and cell lines, correlating with tumor stage and recurrence.
  • RPS9 knockdown inhibited osteosarcoma cell proliferation, colony formation, and induced G1 phase arrest.
  • Knockdown of RPS9 reduced the protein levels of phospho-SAPK/JNK and phospho-p38, indicating MAPK pathway involvement.

Conclusions:

  • RPS9 is upregulated in osteosarcoma and exhibits pro-tumor effects.
  • RPS9 promotes osteosarcoma progression via activation of the MAPK signaling pathway.
  • RPS9 represents a potential therapeutic target for osteosarcoma gene therapy.
Keywords:
MAPK signaling pathwayRPS9osteosarcoma.proliferation

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