Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Active Ran regulates anillin function during cytokinesis.

Daniel Beaudet1, Tara Akhshi2,3, Julia Phillipp1

  • 1Department of Biology, Concordia University, Montreal, QC H4B 1R6, Canada.

Molecular Biology of the Cell
|September 22, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transwell membrane material affects myogenic differentiation in human primary myoblast-adipocyte co-culture.

Physiological reports·2026
Same author

Importin-β1 functions as a chromatin sensor to position the contractile ring for cytokinesis.

Current biology : CB·2026
Same author

Huntingtin polyglutamine expansions misdirect axonal transport by perturbing motor and adaptor recruitment.

iScience·2026
Same author

External mechanical force drives axonal cytoskeletal rearrangements.

Current biology : CB·2026
Same author

Lack of synergy between AR-targeted therapies and PARP inhibitors in homologous recombination-proficient prostate cancer.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Translational changes: evidence for low-dose atropine and myopia control.

Clinical & experimental optometry·2026

Active Ran spatially restricts the actomyosin ring during cytokinesis by regulating anillin localization via importins. This ensures proper cell division and prevents aneuploidy.

Area of Science:

  • Cell biology
  • Molecular biology
  • Genetics

Background:

  • Cytokinesis, the final stage of cell division, requires precise spatial coordination with chromosome segregation to prevent aneuploidy.
  • The mitotic spindle is traditionally thought to control the assembly and constriction of the actomyosin ring during cytokinesis.

Purpose of the Study:

  • To investigate the role of active Ran in spatially regulating cytokinesis.
  • To elucidate the mechanism by which anillin, a key contractile protein, is localized during cell division.

Main Methods:

  • Investigated the effects of altered Ran-GTP levels and Ran localization on anillin.
  • Utilized mutations in anillin's nuclear localization signal (NLS) to assess its cortical affinity and regulation.
  • Examined the role of RhoA-GTP binding in anillin function.

Related Experiment Videos

Main Results:

  • Active Ran gradients, influenced by chromatin position, spatially restrict the actomyosin ring via importin-mediated anillin recruitment.
  • Decreasing Ran-GTP or tethering Ran to the membrane disrupts anillin localization and causes cytokinesis defects.
  • Anillin's NLS is crucial for its cortical localization and function, with RhoA-GTP potentially relieving autoinhibition.

Conclusions:

  • Active Ran acts as a novel spatial regulator of cytokinesis by controlling anillin localization through importins.
  • The NLS-importin interaction in anillin represents a conserved mechanism for regulating contractile ring dynamics.