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Updated: Aug 5, 2026

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
Published on: March 27, 2011
The Nitrate Transporter Family Protein LjNPF8.6 Controls the N-Fixing Nodule Activity
Vladimir Totev Valkov1, Alessandra Rogato1, Ludovico Martins Alves1
1Institute of Biosciences and Bioresources, Institute of Biosciences and Bioresources (IBBR), Consiglio Nazionale delle Ricerche, 80131 Napoli, Italy.
Abstract:
N-fixing nodules are new organs formed on legume roots as a result of the beneficial interaction with soil bacteria, rhizobia. The nodule functioning is still a poorly characterized step of the symbiotic interaction, as only a few of the genes induced in N-fixing nodules have been functionally characterized. We present here the characterization of a member of the Lotus japonicus nitrate transporter1/peptide transporter family, LjNPF8.6 The phenotypic characterization carried out in independent L. japonicus LORE1 insertion lines indicates a positive role of LjNPF8.6 on nodule functioning, as knockout mutants display N-fixation deficiency (25%) and increased nodular superoxide content. The partially compromised nodule functioning induces two striking phenotypes: anthocyanin accumulation already displayed 4 weeks after inoculation and shoot biomass deficiency, which is detected by long-term phenotyping. LjNPF8.6 achieves nitrate uptake in Xenopus laevis oocytes at both 0.5 and 30 mm external concentrations, and a possible role as a nitrate transporter in the control of N-fixing nodule activity is discussed.
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