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Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy
Duncan P Ryan1, Elizabeth A Gould2, Gregory J Seedorf3,4
1Department of Physics, Colorado State University, Fort Collins, CO, 80523, USA.
Nature Communications
|September 22, 2017
Summary
Cleared tissue digital scanned light-sheet microscopy (C-DSLM) overcomes refractive index heterogeneity for in-focus imaging of cleared tissues. This method enables accurate quantification of sub-micron features in large tissue sections.
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