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Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA
D Cross1, M L Allende, R Y Krauss
1Department of Cell Biology, Faculty of Biological Sciences, School of Medicine, Catholic University of Chile, Santiago.
Molecular Biology Reports
|January 1, 1987
Abstract:
In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.