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Hepatocyte Transplantation: Cell Sheet Technology for Liver Cell Transplantation
Kohei Tatsumi1, Teruo Okano2,3
1Department of Physiology and Regenerative Medicine, Kindai University Faculty of Medicine, 377-2 Ohno-higashi, Osaka-sayama, Osaka 589-8511 Japan.
Current Transplantation Reports
|September 22, 2017
Summary
Cell sheet technology offers a promising approach for tissue engineering functional liver tissue. This method has shown success in treating liver diseases and hemophilia in preclinical models.
Area of Science:
- Regenerative Medicine
- Tissue Engineering
- Hepatology
Background:
- Cell-based therapies are emerging for liver disease treatment.
- Hepatocytes are key for liver function.
- Cell sheet technology enables layered cell construct fabrication.
Purpose of the Study:
- Review recent advancements in cell sheet technology for tissue engineering.
- Focus on engineering functional liver tissue using cell sheets.
- Evaluate therapeutic effects for liver diseases, including hemophilia.
Main Methods:
- Development of cell sheet technology for monolithic cell layer harvesting.
- Fabrication of functional liver tissues by stacking primary hepatocyte cell sheets in mice.
- Treatment of hemophilia in mice using cell sheets from genetically modified autologous cells.
Main Results:
- Successful engineering of functional liver tissues in vivo using stacked cell sheets.
- Demonstrated therapeutic efficacy in treating hemophilia mice via cell sheet transplantation.
- Cell sheet technology provides a viable method for creating complex cellular structures.
Conclusions:
- Cell sheet technology presents a feasible and promising strategy for tissue engineering.
- This approach offers new therapeutic avenues for diverse liver diseases.
- Further development holds potential for clinical applications in regenerative medicine.