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Related Concept Videos

Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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Mass spectrometry methods to study protein-metabolite interactions.

Hongbo Guo1, Hui Peng2, Andrew Emili1

  • 1a Donnelly Centre for Cellular and Biomolecular Research , University of Toronto , Toronto , ON , Canada.

Expert Opinion on Drug Discovery
|September 22, 2017
PubMed
Summary

Understanding protein-metabolite interactions is key for biochemical processes. New mass spectrometry methods enable global metabolome-proteome interactome studies, advancing biomedical research and drug discovery.

Keywords:
Protein-metabolite interactionmass spectrometrymetabolomicsproteomics

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Area of Science:

  • Biochemistry
  • Proteomics
  • Metabolomics

Background:

  • Understanding biochemical processes and signaling pathways requires knowledge of endogenous protein-metabolite interactions.
  • Recent advances in mass spectrometry, proteomics, and metabolomics enable global metabolome-proteome interactome studies.

Purpose of the Study:

  • To review innovative mass spectrometry-based methods for elucidating protein-small molecule ligand associations.
  • To highlight tag-free methods, experimental workflows, and data analysis considerations.

Main Methods:

  • Review of mass spectrometry-based chemical-proteomics technologies.
  • Focus on tag-free methods for detecting protein-ligand engagement.
  • Discussion of experimental workflows and computational methods.

Main Results:

  • Emerging technologies facilitate global detection of protein-ligand interactions.
  • Tag-free methods offer promising avenues for interactome studies.
  • Advances in computational methods aid in analyzing complex proteomic and metabolomic data.

Conclusions:

  • Global metabolome-proteome interactome studies are transforming biomedical research and drug discovery.
  • Continued progress in chemical-proteomics is expected to yield new systems biology insights.
  • This field offers new opportunities to improve drug discovery pipelines.