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TPZ, a bright centrosymmetric two-photon scaffold for bioimaging
Zuhai Lei1, Ping Yue, Xueli Wang
1Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. youjunyang@ecust.edu.cn xhqian@ecust.edu.cn.
Chemical Communications (Cambridge, England)
|September 22, 2017
Summary
Researchers developed new biocompatible two-photon fluorophores (TPZ) by modifying PRODAN dye. These novel fluorophores show promise for advanced biological imaging applications in cells, tissues, and zebrafish.
Area of Science:
- Biochemistry
- Organic Chemistry
- Biophysics
Background:
- Developing biocompatible two-photon fluorophores with large absorption cross-sections is difficult.
- Existing theoretical guidelines for fluorophore design are insufficient.
Purpose of the Study:
- To design and synthesize a novel class of two-photon fluorophores (TPZ).
- To investigate the photophysical properties of the new TPZ fluorophores.
- To demonstrate the imaging potential of TPZ in biological systems.
Main Methods:
- Synthesized novel TPZ fluorophores by rendering asymmetric PRODAN dye centrosymmetric.
- Investigated photophysical properties including absorption and emission spectra.
- Evaluated imaging capabilities in cellular, tissue, and whole organism (zebrafish) models.
Main Results:
- Successfully designed and synthesized a new class of TPZ two-photon fluorophores.
- The TPZ fluorophores exhibit favorable photophysical characteristics for imaging.
- Demonstrated effective bioimaging in various biological contexts.
Conclusions:
- The novel TPZ fluorophores offer a promising solution for advanced bioimaging.
- The design strategy of rendering asymmetric dyes centrosymmetric is effective.
- TPZ fluorophores are suitable for in vivo imaging applications.
