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Circular RNA hsa_circ_0001982 Promotes Breast Cancer Cell Carcinogenesis Through Decreasing miR-143

Yi-Yin Tang1, Ping Zhao1, Tian-Ning Zou1

  • 1The First Mammary Surgical Department, The Third Affiliated Hospital of Kunming Medical University and Tumor Hospital of Yunnan Province , Kunming, China .

DNA and Cell Biology
|September 22, 2017
PubMed

Insights

Circular RNAs (circRNAs) play a role in breast cancer. This study found hsa_circ_0001982 promotes tumor growth by sponging miR-143, offering new insights into breast cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are noncoding RNAs involved in tumorigenesis.
  • Their role in breast cancer remains largely unexplored.
  • High-throughput sequencing has revealed numerous circRNAs in tumors.

Purpose of the Study:

  • To investigate circRNA expression profiles in breast cancer tissue.
  • To explore the regulatory mechanisms of circRNAs in breast cancer.
  • To identify specific circRNAs involved in breast cancer progression.

Main Methods:

  • circRNA microarray analysis of breast cancer tissue.
  • Bioinformatics analysis for target prediction.
  • Dual-luciferase reporter assay to confirm interactions.
  • Loss-of-function and rescue experiments in cell lines.

Main Results:

  • 1705 significantly aberrant circRNAs were identified.
  • hsa_circ_0001982 was markedly overexpressed in breast cancer.
  • hsa_circ_0001982 targets miR-143 and promotes proliferation, invasion, and inhibits apoptosis.
  • Knockdown of hsa_circ_0001982 suppressed tumor progression via the miR-143 pathway.

Conclusions:

  • This study provides a preliminary circRNA expression profile in breast cancer.
  • hsa_circ_0001982 acts as a competing endogenous RNA (ceRNA) in breast cancer.
  • The findings offer novel insights into breast cancer tumorigenesis and potential therapeutic targets.
Keywords:
breast cancercircular RNAhsa_circ_0001982miR-143

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