Related Experiment Videos

TFPI2AS1, a novel lncRNA that inhibits cell proliferation and migration in lung cancer

Shenglan Gao1, Ziying Lin1, Chunyan Li1

  • 1a Clinical Research Center, Affiliated Hospital of Guangdong Medical University , Zhanjiang , China.

Cell Cycle (Georgetown, Tex.)
|September 22, 2017
PubMed

Insights

TFPI2AS1, a long non-coding RNA, is upregulated in non-small cell lung cancer (NSCLC). Its knockdown promotes NSCLC cell growth and migration, while its overexpression inhibits these processes, offering new therapeutic insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play critical roles in tumorigenesis, but their specific functions in lung cancer are not fully understood.
  • Identifying novel dysregulated lncRNAs in non-small cell lung cancer (NSCLC) is crucial for understanding cancer progression.

Purpose of the Study:

  • To identify dysregulated lncRNAs in NSCLC using microarray analysis.
  • To investigate the function of the identified lncRNA, TFPI2AS1, in NSCLC cell proliferation and migration.
  • To elucidate the regulatory relationship between TFPI2AS1 and the tumor suppressor TFPI2.

Main Methods:

  • Microarray analysis to identify differentially expressed lncRNAs in NSCLC tissues.
  • In vitro experiments involving TFPI2AS1 knockdown and overexpression in NSCLC cell lines.
  • Cell proliferation and migration assays.
  • Western blotting to assess protein levels (e.g., cyclin D1, CDK2, AKT, TFPI2).

Main Results:

  • TFPI2AS1 was significantly upregulated in NSCLC tumors compared to non-tumor samples.
  • TFPI2AS1 knockdown enhanced NSCLC cell proliferation and migration, linked to G1/S transition and altered cyclin D1/CDK2 levels.
  • TFPI2AS1 overexpression inhibited NSCLC cell proliferation and migration.
  • TFPI2AS1 was found to regulate AKT activation and is a positive regulator of TFPI2 expression.

Conclusions:

  • TFPI2AS1 is a key regulator of NSCLC cell proliferation and migration.
  • TFPI2AS1 exerts its effects, at least in part, by modulating TFPI2 expression.
  • TFPI2AS1 represents a potential therapeutic target for NSCLC.
Keywords:
TFPI2AS1lncRNAlung cancermigrationproliferation

Related Concept Videos

Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...