Complete Genome Sequence of Clostridioides difficile Epidemic Strain DH/NAP11/106/ST-42, Isolated from Stool from a...

Area of Science:

• Microbiology
• Genomics
• Infectious Diseases

Background:

• Clostridioides difficile infection (CDI) is a significant healthcare-associated pathogen.
• Emergence of specific hypervirulent strains like NAP1/BI/027 has been noted.
• Understanding the genomic basis of prevalent strains is crucial for infection control.

Purpose of the Study:

• To report the complete genome sequence of Clostridioides difficile strain DH/NAP11/106/ST-42.
• To provide genomic data for a highly prevalent CDI-causing strain in the U.S.
• To facilitate research into the genetic factors contributing to C. difficile infection virulence and recurrence.

Main Methods:

• Whole-genome sequencing of Clostridioides difficile strain DH/NAP11/106/ST-42.
• Bioinformatic analysis of the obtained genome sequence.
• Isolation of the strain from stool samples of a pediatric patient.

Main Results:

• The complete genome sequence of Clostridioides difficile strain DH/NAP11/106/ST-42 was successfully obtained.
• This strain represents the most common cause of C. difficile infection among U.S. adults.
• The isolate originated from a hospitalized pediatric patient experiencing frequent C. difficile infection relapses.

Conclusions:

• The availability of this genome sequence provides a valuable resource for studying C. difficile.
• Genomic insights may elucidate the factors driving the prevalence and recurrence of this specific C. difficile strain.
• Further research can leverage this data to develop targeted prevention and treatment strategies for CDI.