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Related Experiment Video

Updated: Feb 22, 2026

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications
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Retracing Circulating Tumour Cells for Biomarker Characterization after Enumeration.

Anders S Frandsen1, Anna Fabisiewicz2, Agnieszka Jagiello-Gruszfeld3

  • 1CytoTrack ApS, Lyngby, Denmark.

Journal of Circulating Biomarkers
|September 23, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a novel disc technology for mapping and retracing individual circulating tumor cells (CTCs) from breast cancer patients, enabling future biomarker analysis for personalized cancer therapy.

Keywords:
Breast cancerCKCTCCancerCharacterizationCiculating tumor cellsCytoDiscCytoTrackEnumerationHER2ImmunofluorescenceLiquid biopsyMetastasisMethodRetracing

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Area of Science:

  • Oncology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Individual circulating tumor cell (CTC) analysis is crucial for personalized metastatic cancer therapy, especially when metastasis biopsy is challenging.
  • Biomarker characterization of CTCs aids in understanding cancer progression and tailoring treatments.

Purpose of the Study:

  • To develop and validate a novel disc-based platform for mapping and retracing individual CTCs.
  • To demonstrate the feasibility of downstream biomarker analysis on individually mapped CTCs.

Main Methods:

  • Utilized a novel disc format with the CytoTrack platform to map and retrace individual CTCs from breast cancer patients and nucleated cells from healthy donors.
  • Performed HER2 immunofluorescence characterization on mapped CTCs for proof of concept.

Main Results:

  • Successfully detected and enumerated CTCs in three out of four breast cancer patient samples, with precise mapping on discs.
  • Achieved high recovery rates (96.6%±8.5%) for retracing nucleated cells on discs, with minimal field of view shift (4-29 μm).
  • Demonstrated successful HER2 characterization and retracing of CTCs from a HER2-positive patient, confirming downstream analysis capability.

Conclusions:

  • The developed mapping and retracing technology allows for downstream analysis of individual CTCs for various cancer biomarkers.
  • This novel approach holds significant potential for advancing personalized cancer therapy through detailed CTC analysis.
  • Future research will further explore the capabilities of this retracing technology for biomarker discovery.