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Further evidence of noradrenergic regulation of rat hypothalamic estrogen receptor concentration: possible

J D Blaustein1, J Turcotte

  • 1Neuroscience and Behavior Program and Psychology Department, University of Massachusetts, Amherst 01003.

Brain Research
|December 15, 1987
PubMed
Summary

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The alpha 2-noradrenergic system influences estrogen receptor levels. Yohimbine, an antagonist, initially increased cytosol estrogen receptors, but this was not a functional increase, followed by a later decrease.

Area of Science:

  • Neuroendocrinology
  • Pharmacology

Background:

  • Estrogen receptors (ERs) are crucial for reproductive functions.
  • The alpha-noradrenergic system modulates various physiological processes, including hormone regulation.

Purpose of the Study:

  • To investigate the role of the alpha 2-noradrenergic system in regulating estrogen receptor concentration.
  • To elucidate the biphasic effects of alpha 2-noradrenergic antagonists on ER levels in the hypothalamus and pituitary.

Main Methods:

  • Administration of alpha 2-noradrenergic antagonist (yohimbine) and agonist (clonidine).
  • Measurement of cytosol and cell nuclear estrogen receptor concentrations.
  • Scatchard analysis to assess receptor binding characteristics.

Main Results:

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  • Yohimbine induced a transient increase in cytosol ER concentration, not affecting receptor affinity.
  • This initial increase did not lead to increased cell nuclear ER accumulation.
  • A subsequent, prolonged decrease in cytosol ER concentration was observed, correlating with reduced nuclear ER accumulation after estradiol challenge.

Conclusions:

  • The alpha 2-noradrenergic system, particularly the alpha 2-subtype, appears to decrease estrogen receptor levels in the brain and pituitary.
  • This interaction may represent a mechanism for environmental regulation of neuronal sensitivity to estradiol.
  • The physiological relevance of the initial, non-functional increase in cytosol ER concentration requires further investigation.