Nickel accumulation by the green algae-like Euglena gracilis

Nickel accumulation and nickel effects on cellular growth, respiration, photosynthesis, ascorbate peroxidase (APX) activity, and levels of thiols, histidine and phosphate-molecules were determined in Euglena gracilis. Cells incubated with 0.5-1mM NiCl₂ showed impairment of O₂ consumption, photosynthesis, Chl a+b content and APX activity whereas cellular integrity and viability were unaltered. Nickel accumulation was depressed by Mg²⁺ and Cu²⁺, while Ca²⁺, Co²⁺, Mn²⁺ and Zn²⁺ were innocuous. The growth half-inhibitory concentrations for Ni²⁺ in the culture medium supplemented with 2 or 0.2mM Mg²⁺ were 0.43 or 0.03mM Ni²⁺, respectively. Maximal nickel accumulation (1362mg nickel/Kg DW) was achieved in cells exposed to 1mM Ni²⁺ for 24h in the absence of Mg²⁺ and Cu²⁺; accumulated nickel was partially released after 72h. GSH polymers content increased or remained unchanged in cells exposed to 0.05-1mM Ni²⁺; however, GSH, cysteine, γ-glutamylcysteine, and phosphate-molecules all decreased after 72h. Histidine content increased in cells stressed with 0.05 and 0.5mM Ni²⁺ for 24h but not at longer times. It was concluded that E. gracilis can accumulate high nickel levels depending on the external Mg²⁺ and Cu²⁺ concentrations, in a process in which thiols, histidine and phosphate-molecules have a moderate contribution.