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UbasM: An effective balanced optical clearing method for intact biomedical imaging.

Lingling Chen1, Guiye Li2, Yamin Li2

  • 1College of Optoelectronics Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, P. R. China. l.chen10@szu.edu.cn.

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Summary
This summary is machine-generated.

We developed UbasM, a fast and inexpensive aqueous optical clearing agent. This method enables deep tissue imaging while preserving fluorescent signals and tissue integrity for biological research.

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Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Tissue Clearing

Background:

  • Optical clearing enhances deep tissue imaging by reducing light scattering.
  • Existing methods compromise clearing efficiency, fluorescent signal preservation, membrane integrity, or speed.
  • Slow processing times (weeks) limit scalability for large tissue samples.

Purpose of the Study:

  • Introduce a novel, rapid, aqueous optical clearing agent (UbasM).
  • Address limitations of current clearing techniques for biological tissues.
  • Enable efficient, high-fidelity, large-scale tissue imaging.

Main Methods:

  • Development of UbasM (Urea-Based Amino-Sugar Mixture), an aqueous clearing solution.
  • Testing UbasM's clearing capability on fixed biological tissues.
  • Assessing preservation of fluorescent protein and lipophilic dye signals.
  • Evaluating membrane integrity post-clearing.
  • Assessing processing speed for cm-scale samples.

Main Results:

  • UbasM rapidly renders fixed tissues highly transparent.
  • Reliable preservation of fluorescent protein and lipophilic dye emission was achieved.
  • Membrane integrity was maintained in cleared tissues.
  • UbasM is simple, inexpensive, reproducible, and compatible with various labeling methods.
  • Enabled volumetric imaging of tissues up to whole adult mouse organs.

Conclusions:

  • UbasM offers a rapid, effective, and versatile solution for optical tissue clearing.
  • Facilitates deep, high-resolution imaging of large biological samples.
  • Supports diverse microscopy and tomography techniques for systems biology research.