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Updated: Feb 22, 2026

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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
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Fluorescence Microscopy of Nanochannel-Confined DNA
Fredrik Westerlund1, Fredrik Persson2, Joachim Fritzsche1
1Chalmers University of Technology, Gothenburg, Sweden.
Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2017
Abstract:
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

