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Updated: Feb 22, 2026

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
Published on: June 19, 2017
Erratum to: Discovery of two small circular ssDNA viruses associated with the whitefly Bemisia tabaci
Erich Y T Nakasu1, Fernando L Melo2, Miguel Michereff-Filho1
1Embrapa Vegetables, Rodovia BR-060, Km09, Brasília, 70275-970, Brazil.
Abstract:
The complete genome sequences of two novel small circular DNA viruses isolated from sweet-potato whiteflies collected in Central-West (AdDF) and Southeast (AdO) regions of Brazil were determined by Next Generation Sequencing (NGS), and confirmed by cloning and Sanger sequencing. The genomes are 2,199 and 2,211 nt-long, respectively, encoding a putative coat protein (CP) and a replication-associated protein (Rep) and showing a genomic organization typical of viruses from the family Genomoviridae. Phylogenetic analysis with deduced amino acid sequences of Rep indicates that the virus from AdO is closely related to other members of the genus Gemycircularvirus, while the virus from AdDF is related to those of the genus Gemyduguivirus. These new genomoviruses are tentatively named bemisia-associated genomovirus AdO and bemisia-associated genomovirus AdDF.

