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Summary
This summary is machine-generated.

This study introduces a novel, reversible glucose sensor using glucose oxidase-wrapped single-walled carbon nanotubes (SWCNTs). This mediatorless biosensor shows potential for continuous glucose monitoring due to its selective near-infrared fluorescence response.

Keywords:
glucoseglucose oxidasenear-infrared (NIR) fluorescenceoptical sensorssingle-walled carbon nanotubes (SWCNTs or SWNTs)

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Single-walled carbon nanotubes (SWCNTs) offer unique near-infrared fluorescence, photostability, and tissue transparency for in vivo biosensing.
  • Current SWCNT optical sensors often require exogenous mediators, limiting sensor stability and biocompatibility.
  • Developing mediatorless biosensors is crucial for advanced in vivo applications.

Purpose of the Study:

  • To develop a reversible, mediatorless near-infrared glucose sensor using glucose oxidase-wrapped SWCNTs (GOx-SWCNTs).
  • To investigate the fluorescence enhancement mechanism and sensor performance for glucose detection.
  • To assess the sensor's suitability for continuous glucose monitoring.

Main Methods:

  • Fabrication of glucose oxidase-wrapped SWCNTs (GOx-SWCNTs).
  • Characterization using fluorescence, absorption, and Raman spectroscopy.
  • Integration into a custom-built membrane device for performance evaluation.
  • Testing sensor response to varying glucose concentrations and reversibility.

Main Results:

  • GOx-SWCNTs exhibited selective fluorescence enhancement in the presence of aldohexoses, particularly glucose.
  • The sensor demonstrated a monotonic response to glucose concentrations ranging from 3 × 10⁻³ to 30 × 10⁻³ M.
  • A fluorescence enhancement mechanism involving localized enzymatic doping of SWCNT defect sites was proposed.
  • Complete fluorescence recovery upon glucose removal confirmed sensor reversibility.

Conclusions:

  • The developed GOx-SWCNT sensor is a reversible, mediatorless system for near-infrared glucose detection.
  • The sensor's mechanism relies on enzymatic doping, avoiding the need for external mediators.
  • The demonstrated reversibility is a key feature for potential continuous glucose monitoring applications.