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Lanthanide-Coordinated Semiconducting Polymer Dots Used for Flow Cytometry and Mass Cytometry
Xu Wu1, Quinn DeGottardi2,3, I-Che Wu1
1Department of Chemistry, University of Washington, Seattle, WA, 98195, USA.
Angewandte Chemie (International Ed. in English)
|September 24, 2017
Summary
Researchers developed novel polymer dots (Pdots) for simultaneous biomarker monitoring. These dual-functionality probes enhance both flow cytometry and mass cytometry analyses, improving disease and drug discovery research.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Cell Biology
Background:
- Simultaneous biomarker monitoring and single-cell analysis are crucial for understanding disease mechanisms, drug discovery, and cellular signaling networks.
- Flow cytometry and mass cytometry are powerful, complementary single-cell analysis techniques, but require probes with specific properties.
- Existing probes often have limitations, hindering their effectiveness across both flow and mass cytometry platforms.
Purpose of the Study:
- To develop and validate a novel dual-functionality probe for simultaneous use in flow cytometry and mass cytometry.
- To address the limitations of current probes for advanced single-cell analysis techniques.
- To demonstrate the probe's utility in complex biological samples.
Main Methods:
- Synthesis of lanthanide-coordinated semiconducting polymer dots (Pdots) exhibiting both fluorescence and mass signals.
- Application of the dual-functionality Pdots in flow cytometry for fluorescence detection.
- Utilized the Pdots in mass cytometry for high-sensitivity mass signal detection.
- Tested the probes in mimetic cell mixtures and human peripheral blood mononuclear cells.
Main Results:
- The developed Pdots possess both fluorescence and mass signals, enabling dual-mode detection.
- The Pdots demonstrated high fluorescence signals suitable for flow cytometry applications.
- In mass cytometry, the Pdots exhibited superior performance compared to commercially available probes.
- Successful application in both model systems, validating their utility.
Conclusions:
- Lanthanide-coordinated semiconducting polymer dots offer a versatile solution for dual-mode flow and mass cytometry.
- These novel probes enhance the capabilities of single-cell analysis, advancing disease mechanism and drug discovery research.
- The Pdots provide a promising alternative to existing probes, offering improved performance in mass cytometry.
