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Potiskum virus: enhancement of replication in a macrophage-like cell line
A H Fagbami1, S B Halstead, N Marchette
1Department of Virology, College of Medicine, University of Ibadan, Nigeria.
Abstract:
Replication of Potiskum virus was studied in P388D1 macrophage-like cell line in the presence and absence of subneutralizing concentrations of specific antiviral antibody. The cultures were infected at multiplicities of infection (MOI) ranging from 0.4 to 0.0004. The virus replicated to high titres at all MOI tested, but there was an enhancement of virus replication in cultures supplemented with the antibody. Enhancement of replication was MOI dependent, the highest ratios being obtained in cultures infected at lowest MOI. In enhancement assays using various dilutions of immune mouse ascitic fluid (IMAF), the highest enhancement ratio was observed at dilution 1 : 500; the enhancing antibody titre was 5,000.
Insights
Potiskum virus replication in macrophage cells was enhanced by specific antiviral antibodies. This antibody-mediated enhancement was most pronounced at lower multiplicities of infection (MOI).
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Potiskum virus is a pathogen that infects macrophage-like cell lines.
- Antiviral antibodies can modulate viral replication.
- Understanding virus-antibody interactions is crucial for developing effective antiviral strategies.
Purpose of the Study:
- To investigate the effect of subneutralizing concentrations of specific antiviral antibody on Potiskum virus replication.
- To determine the influence of multiplicity of infection (MOI) on antibody-mediated enhancement of viral replication.
Main Methods:
- Potiskum virus replication was studied in P388D1 macrophage-like cells.
- Cells were infected at various multiplicities of infection (MOI) ranging from 0.4 to 0.0004.
- The effect of specific antiviral antibody (immune mouse ascitic fluid - IMAF) at different dilutions was assessed.
Main Results:
- Potiskum virus replicated to high titers across all tested MOI.
- Supplementation with specific antiviral antibody significantly enhanced virus replication.
- The enhancement of replication was dependent on MOI, with the greatest effect observed at the lowest MOI.
- The highest enhancement ratio was observed at a 1:500 dilution of IMAF, with an enhancing antibody titre of 5,000.
Conclusions:
- Subneutralizing concentrations of specific antiviral antibodies can enhance Potiskum virus replication in macrophage-like cells.
- This enhancement is dependent on the multiplicity of infection, being more significant at lower MOI.
- These findings highlight a complex interplay between Potiskum virus, host cells, and the immune response, potentially impacting viral pathogenesis and control strategies.