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Amentoflavone suppresses tumor growth in ovarian cancer by modulating Skp2

Honggai Liu1, Qingfen Yue1, Shehong He1

  • 1Department of Gynaecology, Luoyang Central Hospital, Zhengzhou University, China.

Life Sciences
|September 25, 2017
PubMed
Abstract

Insights

Amentoflavone (AF) effectively suppresses ovarian cancer cell growth and promotes apoptosis. This natural compound works by downregulating Skp2 expression via the ROS/AMPK/mTOR pathway, showing therapeutic potential for ovarian cancer treatment.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Ovarian cancer is a leading cause of cancer-related deaths in women, characterized by high recurrence rates and poor prognosis.
  • Amentoflavone (AF), a natural compound from S. tamariscina, is investigated for its anti-cancer properties.

Purpose of the Study:

  • To investigate the anti-tumor effects of amentoflavone (AF) against human ovarian cancer cell lines.
  • To elucidate the underlying molecular mechanisms of AF's anti-cancer activity.

Main Methods:

  • Utilized human ovarian cancer cell lines (SKOV3, OVCAR-3) and a xenograft mouse model.
  • Assessed cell viability (CCK-8), apoptosis and cell cycle (flow cytometry), ROS generation, and protein expression (Western blot).

Main Results:

  • AF significantly inhibited ovarian cancer cell proliferation, induced apoptosis, and caused cell cycle arrest.
  • AF downregulated S-phase kinase protein 2 (Skp2) expression, a key mechanism for its anti-tumor effect.
  • AF repressed Skp2 expression through the ROS/AMPK/mTOR signaling pathway, confirmed in vivo.

Conclusions:

  • Amentoflavone (AF) demonstrates significant potential as a therapeutic agent for ovarian cancer.
  • The study elucidates AF's anti-tumor mechanisms involving Skp2 downregulation via ROS/AMPK/mTOR signaling.
Keywords:
AMPKAmentoflavoneOvarian cancerSkp2

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