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DNA extraction on bio-chip: history and preeminence over conventional and solid-phase extraction methods
Adilah Ayoib1, Uda Hashim2, Subash C B Gopinath3,4
1Ganoderma Research Group, Institute of Nano Electronic Engineering, University Malaysia Perlis, 01000, Kangar, Malaysia. adilahayoib@gmail.com.
Applied Microbiology and Biotechnology
|September 25, 2017
Summary
This review traces the evolution of biological classification from cellular to DNA-level analysis. It highlights advances in deoxyribonucleic acid (DNA) extraction methods, from conventional techniques to modern microfluidics, crucial for future biological insights.
Area of Science:
- Evolution of biological classification
- Advancements in molecular biology techniques
- Biotechnology and analytical methods
Background:
- Early biological classification relied on cellular morphology.
- The advent of electron microscopy revolutionized cellular studies.
- Deoxyribonucleic acid (DNA) analysis enabled classification at a molecular level.
Purpose of the Study:
- To review the developmental progression of taxonomy from cellular to DNA-based classification.
- To discuss the evolution of DNA extraction methodologies.
- To highlight the significance of Lab-on-a-Chip (LOC) systems in future biological classification.
Main Methods:
- Review of conventional chemical DNA extraction methods, including liquid/liquid extraction (LLE).
- Discussion of solid-phase extraction (SPE) methods.
- Exploration of microfluidics device-based systems for on-chip DNA extraction.
Main Results:
- Conventional chemical methods provide fundamental DNA extraction capabilities.
- Solid-phase extraction (SPE) offers advancements over LLE.
- Microfluidics and Lab-on-a-Chip (LOC) systems represent the future of efficient DNA extraction.
Conclusions:
- DNA extraction is pivotal for modern biological classification.
- Lab-on-a-Chip (LOC) systems offer significant advantages over traditional methods.
- Technological advancements in DNA extraction are crucial for future biological research and classification.
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