THE SYNTHESIS OF ¹³C₉-¹⁵N-LABELED 3,5-DIIODOTHYRONINE AND THYROXINE

Area of Science:

• Biochemistry
• Endocrinology
• Analytical Chemistry

Background:

• Thyroid hormones are crucial for regulating metabolic activity.
• Understanding thyroid hormone metabolism is vital for physiological and pathological studies.
• Existing radiolabeled hormones have limitations for certain analytical techniques.

Purpose of the Study:

• To synthesize stable isotope labeled thyroid hormone derivatives.
• To enable in vivo studies of thyroid hormone metabolism using LC-MS/MS.
• To provide a non-radioactive alternative for hormone tracking.

Main Methods:

• Chemical synthesis of stable isotope labeled thyroid hormones.
• Incorporation of 13C and 15N isotopes into T2 and T4 structures.
• Preparation for use in Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) analyses.

Main Results:

• Successful synthesis of 13C9-15N-T2 and 13C9-15N-T4.
• These labeled compounds are suitable for in vivo applications.
• The synthesized hormones are designed for LC-MS/MS quantification.

Conclusions:

• Stable isotope labeled thyroid hormones (13C9-15N-T2 and 13C9-15N-T4) have been synthesized.
• These labeled hormones are valuable tools for in vivo LC-MS/MS studies of thyroid hormone metabolism.
• This advancement offers a non-radioactive method for detailed metabolic pathway analysis.