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Related Experiment Video

Updated: Feb 22, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

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A robust, single-injection method for targeted, broad-spectrum plasma metabolomics.

Kefeng Li1,2, Jane C Naviaux1,3, A Taylor Bright1,2

  • 1The Mitochondrial and Metabolic Disease Center, University of California, San Diego, School of Medicine, 214 Dickinson St., Bldg CTF, Rm C102, San Diego, CA 92103-8467 USA.

Metabolomics : Official Journal of the Metabolomic Society
|September 26, 2017
PubMed
Summary

A new targeted metabolomic method measures over 600 plasma metabolites in one injection. This broad-spectrum approach shows promise for diagnosing complex diseases like chronic fatigue syndrome (CFS).

Keywords:
Broad-spectrumChronic fatigue syndromeHydrophilic interaction chromatographyLC-MS/MSTargeted metabolomicsValidation

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Last Updated: Feb 22, 2026

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Area of Science:

  • Biochemistry
  • Systems Biology
  • Analytical Chemistry

Background:

  • Metabolomics offers powerful insights into health and disease.
  • Current targeted methods face limitations in analyte number and require multiple injections.

Purpose of the Study:

  • To develop a single-injection, targeted, broad-spectrum plasma metabolomic method.
  • To validate the method's analytical performance and clinical utility for chronic fatigue syndrome (CFS) diagnosis.

Main Methods:

  • A SCIEX Qtrap 5500 LC-ESI-MS/MS platform was utilized.
  • Analytical validation included reproducibility, linearity, carryover, and blood collection tube effects.
  • Clinical validation involved a cohort of 22 male CFS patients and 18 controls.

Main Results:

  • The method measures 610 metabolites from 63 pathways and 95 standards in 45 minutes via a single injection.
  • Total imprecision (CVtotal) of peak area was 12% for control and CFS pools.
  • Clinical validation demonstrated 95% diagnostic accuracy for CFS using 8 key metabolites.

Conclusions:

  • A reliable, reproducible, broad-spectrum targeted metabolomic method was developed.
  • The method can measure over 600 plasma metabolites in a single injection.
  • This technique may aid in diagnosing CFS and other complex diseases.