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"Open-top" microfluidic device for in vitro three-dimensional capillary beds.

Soojung Oh1, Hyunryul Ryu, Dongha Tahk

  • 1School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, South Korea.

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Summary

This study presents an innovative open-top microfluidic device for co-culturing blood vessels and tissues. The device enables long-term culture and mimics in vivo conditions, facilitating new vascularized tissue models.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Tissue Engineering

Background:

  • Developing in vivo-like niche models for vascularized tissues is crucial for disease research.
  • Existing microfluidic devices face limitations in long-term culture and co-culturing complex biological systems.

Purpose of the Study:

  • To introduce a novel open-top microfluidic device for co-culturing blood vessel networks and cell tissues.
  • To demonstrate the device's capability in mimicking in vivo vascular niche conditions.
  • To explore the interaction between microvessels and cancer spheroids within the device.

Main Methods:

  • Design and fabrication of an "open-top" microfluidic device with micropores for fluid access.
  • Co-culturing of large-scale microvessel networks and small cell tissues.
  • Mimicking capillary bed conditions by co-culturing microvessels with cancer spheroids.

Main Results:

  • Successful assembly of microvessels with 5 mm channel widths.
  • Demonstrated long-term culture of microvessel networks.
  • Observed vessel recruitment and increased vessel formation upon interaction with cancer spheroids.
  • Noted alterations in microvessel morphology due to cancer spheroid proximity.

Conclusions:

  • The open-top microfluidic device offers novel advantages for vascularized tissue engineering.
  • The device successfully mimics in vivo-like niche conditions for studying microvessel-tissue interactions.
  • This platform holds significant potential for developing advanced vascularized tissue models for research and drug testing.